ISOLATION OF A SOLUBLE TRANSPLANTATION ANTIGEN

Electrophoretic purification of a water-soluble guinea pig transplantation antigen.

The isolation of tissue transplantation antigens has been energetically pursued since Billingham et al.' found that cell extracts could induce homograft immunity. A major problem in this endeavor has been the solubilization of the antigens which probably reside on cellular membranes. Studies employing tissue homogenization' and pressure decompression' to disrupt cells have yielded insoluble ant...

Induction of transplantation resistance with soluble simian virus 40-induced hamster tumor-specific transplantation antigen.

identifying their role in tumor progression or rejection. The history of modern efforts to prepare cell-free, membrane-as sociated TSTA's goes back almost 2 decades now, and the chemical identity of purified TSTA in any system is still un known. The early attempts to isolate TSTA were fraught with disap pointment in the oncodnavirus model system in hamsters (11). The typical finding was that an...

Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures

Alloreactive immune responses of transgenic mice expressing a foreign transplantation antigen in a soluble form.

Transfection of cells with the H-2Kk gene lacking the transmembrane and cytoplasmic segments resulted in secretion of the H-2Kk protein, as determined by immunoprecipitation with monoclonal anti-H-2Kk antibodies. Transgenic (H-2b X H-2d)F1 mice were established carrying integrated copies of the modified H-2Kk gene. Expression of the soluble H-2Kk antigen in the transgenic mice was demonstrated ...

Isolation of a new soluble antigen from the yeast phase of Histoplasma capsulatum.

A method is described by which a soluble antigen was prepared from the yeast phase of Histoplasma capsulatum. This soluble preparation had a specificity greater than that of whole-cell yeast-phase antigens. In complement fixation tests with sera from human cases of histoplasmosis, blastomycosis, and coccidioidomycosis, the soluble antigen reacted in 12.1% of 141 tests with heterologous sera, wh...